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Image Search Results
Journal: Nucleic Acids Research
Article Title: Protein–protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy
doi: 10.1093/nar/gkl477
Figure Lengend Snippet: Materials for fluorescence labeling. ( A ) The structure of a fluorescent puromycin derivative. A fluorophore (Cy5 or RhG) and iminobiotin were chemically conjugated to puromycin through a linker. ( B ) DNA construction for fluorescence labeling of proteins. Template DNA consists of SP6 promoter, Omega sequence and an open reading frame (ORF) with a T7·tag at the N-terminus and a polyhistidine tag at the C-terminus, followed by a XhoI restriction enzyme site.
Article Snippet: The purified DNA was transcribed in an
Techniques: Fluorescence, Labeling, Sequencing